|The Cape Peninsula University of Technology (CPUT) Electronic Theses and Dissertations (ETD) repository holds full-text theses and dissertations submitted for higher degrees at the University (including submissions from former Cape Technikon and Peninsula Technikon).|
An in vitro comparison of cellular destruction and metabolic effects occurring in stored, leuco-reduced and irradiated red blood cells
Biochemical and haematological changes occur in red blood cellular products during the recommended storage period of 35 to 42 days at 1°C to 6°C. The restriction of the sodium/potassium pump at specified temperatures result in low intracellular potassium ion levels while an increase in sodium ion levels are observed and acidosis occurs as a result of low pH concentrations due to glucose consumption. Structural and morphological changes occur such as the release of free haemoglobin, lactate dehydrogenase and potassium into the supernatant causing the formation of spheroechinocytes and osmotic fragility. All these factors negatively impact the rheological properties of blood. These changes that transpire in the red cells during the storage period are referred to as “storage lesions”. Transfusion-associated graft versus host disease is an immunological and often fatal adverse transfusion reaction with gamma irradiation of cellular blood products used as a preventative measure. Gamma irradiation exacerbates storage lesions and of particular concern has been the increased potassium levels resulting in neonatal and infant hyperkalaemia. The storage lesions occurring in non-irradiated red blood cellular products are well documented although the literature regarding its irradiated counterparts has been less studied. A study of this nature has not previously been done in Cape Town, South Africa.
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-sa/3.0/za/
Showing items related by title, author, creator and subject.
Effect of rooibos and red palm oil supplementation, alone or in combination, on cardiac function after exposure to hypertension and inflammation in an ischaemial/reperfusion injury model Thamahane-Katengua, Emma Tutu Masechela (Cape Peninsula University of Technology, 2013)Cardiovascular disease (CVD) is without a doubt one of the most challenging health issues of our time and accounts for the highest number of deaths in both developed and developing countries. Despite the huge strides that ...
Evaluation of the labelling and binding efficiency of Tc-99m to red blood cells of patients who are being exposed to a cocktail of anti-tuberculosis drugs Lackay, Carolynn Louise (Cape Peninsula University of Technology, 2010)Radioactively labelled red blood cells (RBC) are used in various nuclear medicine studies. In order to obtain accurate results when performing these studies, it is of paramount importance that a good binding of the ...
Die bepaling van sekere plaagdoderreste in die bloed van plaaswerkers op appelplase in die Elgin-distrik Morren, Carel-Jan Hendrikus (Cape Technikon, 1994)Pesticides are generally used in south-Africa for the control of various pests; from insects and fungi to weeds. The agricultural industry is probably the biggest user of pesticides and therefore workers in this part ...