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Bioassay-guided detection of antimicrobial components from Sutherlandia frutescens and Salvia Africana-lutea
Author(s)
Obidigbo, Chidimma Cynthia Esther
Date Issued
2024
Type
Thesis
Publisher
Cape Peninsula University of Technology
Abstract
Plants remain beneficial to humans and have been used as a source of medicine for various
ailments including infections. Salvia africana-lutea and Sutherlandia frutescens are endemic
to South Africa and have been traditionally used to treat and manage a wide variety of
infections including respiratory, urinary tract and skin infections, Human Immunodeficiency
Virus, coughs and cancer. Many scientific studies have confirmed some of these medicinal
activities.
The current study sought to investigate antibacterial properties of Salvia africana-lutea and
Sutherlandia frutescens. To determine the respective minimum inhibitory concentrations, a
thorough investigation of antibacterial activity against bacteria such as Staphylococcus
aureus, Methicillin-resistant Staphylococcus aureus, Staphylococcus epidermidis and
Pseudomonas aeruginosa was carried out using agar well-diffusion and microdilution assays.
Results indicate that Salvia africana-lutea extracts have better antibacterial activity in
comparison to Sutherlandia frutescens extracts. The minimum inhibitory concentrations of
Salvia africana-lutea extract ranged from 4 to 10mg/ml. When compared to Sutherlandia
frutescens, which had very poor antibacterial activity; the aqueous, ethanol, and acetone
extracts from Salvia africana-lutea showed good antibacterial activity against Staphylococcus
aureus, Methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis. Salvia
africana-lutea extracts however had little to no antibacterial activity against Pseudomonas
aeruginosa.
Phytochemical techniques, such as Thin-Layer Chromatography, Bioautography, Nuclear
Magnetic Resonance Spectrometry, and Liquid-Chromatography Mass Spectrometry were
employed to detect and possibly identify the active fractions/compounds. A few compounds
(relative to rosmarinic acid) were identified in acetone and ethanol extracts of Salvia africanalutea.
By identifying active compounds against medically significant microbes, this study adds
a layer of gravitas to the already existing knowledge on medicinal properties of Salvia africanalutea.
Outputs of this research may provide leads to possible new drug formulations and
preparations that might help control and reduce the burden of infections.
ailments including infections. Salvia africana-lutea and Sutherlandia frutescens are endemic
to South Africa and have been traditionally used to treat and manage a wide variety of
infections including respiratory, urinary tract and skin infections, Human Immunodeficiency
Virus, coughs and cancer. Many scientific studies have confirmed some of these medicinal
activities.
The current study sought to investigate antibacterial properties of Salvia africana-lutea and
Sutherlandia frutescens. To determine the respective minimum inhibitory concentrations, a
thorough investigation of antibacterial activity against bacteria such as Staphylococcus
aureus, Methicillin-resistant Staphylococcus aureus, Staphylococcus epidermidis and
Pseudomonas aeruginosa was carried out using agar well-diffusion and microdilution assays.
Results indicate that Salvia africana-lutea extracts have better antibacterial activity in
comparison to Sutherlandia frutescens extracts. The minimum inhibitory concentrations of
Salvia africana-lutea extract ranged from 4 to 10mg/ml. When compared to Sutherlandia
frutescens, which had very poor antibacterial activity; the aqueous, ethanol, and acetone
extracts from Salvia africana-lutea showed good antibacterial activity against Staphylococcus
aureus, Methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis. Salvia
africana-lutea extracts however had little to no antibacterial activity against Pseudomonas
aeruginosa.
Phytochemical techniques, such as Thin-Layer Chromatography, Bioautography, Nuclear
Magnetic Resonance Spectrometry, and Liquid-Chromatography Mass Spectrometry were
employed to detect and possibly identify the active fractions/compounds. A few compounds
(relative to rosmarinic acid) were identified in acetone and ethanol extracts of Salvia africanalutea.
By identifying active compounds against medically significant microbes, this study adds
a layer of gravitas to the already existing knowledge on medicinal properties of Salvia africanalutea.
Outputs of this research may provide leads to possible new drug formulations and
preparations that might help control and reduce the burden of infections.
Additional information
Thesis (MSc (Biomedical Technology))--Cape Peninsula University of Technology, 2024
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