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The use of fluorescent flow cytometry to evaluate the inactivation of Saccharomyces cerevisiae by sequential application of ultrsound (20kHz) and heat
Author(s)
Wordon, Brett Arthur
Date Issued
2009
Type
Thesis
Publisher
Cape Peninsula University of Technology
Abstract
The primary aim of this study was to establish the effects of both cavitation, (20 KHZ), and
heat (55°C or 60•C) on Saccharomyces cerevisiae GC210 (MATa lys2) suspended in
physiological saline. Fluorescent flow cytometry was used to determine the effects of moist
heat and acoustic cavitation on S. cerevisiae cells. Results from this study could be used as
a guide for use by the food industry for the combined use of heat and sonication to disinfect
various solutions contaminated with S. cerevisiae.
heat (55°C or 60•C) on Saccharomyces cerevisiae GC210 (MATa lys2) suspended in
physiological saline. Fluorescent flow cytometry was used to determine the effects of moist
heat and acoustic cavitation on S. cerevisiae cells. Results from this study could be used as
a guide for use by the food industry for the combined use of heat and sonication to disinfect
various solutions contaminated with S. cerevisiae.
Additional information
Thesis (MTech (Food Technology)--Cape Peninsula University of Technology, 2009
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199011605_Wordon_BA_2009.pdf
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