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Validation and application of the ELISA technique for the detection of fish aero-antigens
Author(s)
George, Dashwill Anton
Date Issued
2003
Type
Thesis
Publisher
Peninsula Technikon
Abstract
Increased seafood consumption due to its nutrition and promotion of a healthy
diet has lead to more frequent reports of allergic reactions. In the seafood
industry, workers are exposed to the antigens through inhalation of the vapours
created during the seafood processing and cooking. Most seafood allergens are
stable molecules, which are resistant to the effect of cooking and processing.
The prevalence of occupational asthma varies from 7-36% among different
groups of workers including seafood processing and fishmeal workers,
fishermen and restaurant cooks (Jeebhay et al 2001).
Purpose of Study:
The purpose of the study is to determine total protein and the specific fish
antigen concentrations in the environment by means of personal air sampling
filters obtained from various categories of workers in the seafood processing
industry.
Objectives:
• To determine the correlation between total protein concentrations and
specific fish (pilchard and anchovy) antigen concentrations on personal
air sampling filters using the linear response model of the standard
curve.
• To determine the correlation between total protein concentrations and
specific fish (pilchard and anchovy) antigen concentrations on personal
air sampling filters using the sigmoidal response model with a variable
slope of the standard curve.
• To identify the most efficient standard curve response model for fish
antigen detection by comparing the percentage recovery of the linear
standard curve response model and the sigmoidal standard curve
response model.
Methodology:
A sample population of 195 samples was taken from workers in the seafood
industry at the St. Helena Bay Fisheries and West Point Processors using
personal air sampling pumps.
diet has lead to more frequent reports of allergic reactions. In the seafood
industry, workers are exposed to the antigens through inhalation of the vapours
created during the seafood processing and cooking. Most seafood allergens are
stable molecules, which are resistant to the effect of cooking and processing.
The prevalence of occupational asthma varies from 7-36% among different
groups of workers including seafood processing and fishmeal workers,
fishermen and restaurant cooks (Jeebhay et al 2001).
Purpose of Study:
The purpose of the study is to determine total protein and the specific fish
antigen concentrations in the environment by means of personal air sampling
filters obtained from various categories of workers in the seafood processing
industry.
Objectives:
• To determine the correlation between total protein concentrations and
specific fish (pilchard and anchovy) antigen concentrations on personal
air sampling filters using the linear response model of the standard
curve.
• To determine the correlation between total protein concentrations and
specific fish (pilchard and anchovy) antigen concentrations on personal
air sampling filters using the sigmoidal response model with a variable
slope of the standard curve.
• To identify the most efficient standard curve response model for fish
antigen detection by comparing the percentage recovery of the linear
standard curve response model and the sigmoidal standard curve
response model.
Methodology:
A sample population of 195 samples was taken from workers in the seafood
industry at the St. Helena Bay Fisheries and West Point Processors using
personal air sampling pumps.
Additional information
Thesis (MTech (Biomedical Technology))--Peninsula Technikon, 2003
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Validation and application of the Elisa technique.pdf
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