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Assay of glutamine synthetase in cerebrospinal fluid as a specific marker in Alzheimer's disease
Author(s)
Oettle, Nicola
Date Issued
1997
Type
Thesis
Publisher
Cape Technikon
Abstract
There is, at present, no recognised diagnostic biochemical
marker of Alzheimer's Disease (AD). Recently, Gunnerson and
Haley, (1992), reported that the presence of glutamine
synthetase (GS) in cerebrospinal fluid (CSF) samples showed a
97% correlation with patients diagnosed as having AD. GS was
detected by photolabelling with [y32P]2-azido-ATP or [y32P]8azido-
ATP and visualisation following sodium dodecyl sulphate
polyacrylamide gel electrophoresis (SOS-PAGE) and
autoradiography.
This study set out to reproduce Gunnerson and Haley's
methodology for labelling sheep GS in CSF using [y32P]8-azidoATP,
to develop this assay or possibly another, using a
fluorescent probe of ATP binding sites, into a robust
procedure suitable for a routine diagnostic laboratory, and
finally to assess whether the presence of GS in CSF is indeed
a marker of AD.
marker of Alzheimer's Disease (AD). Recently, Gunnerson and
Haley, (1992), reported that the presence of glutamine
synthetase (GS) in cerebrospinal fluid (CSF) samples showed a
97% correlation with patients diagnosed as having AD. GS was
detected by photolabelling with [y32P]2-azido-ATP or [y32P]8azido-
ATP and visualisation following sodium dodecyl sulphate
polyacrylamide gel electrophoresis (SOS-PAGE) and
autoradiography.
This study set out to reproduce Gunnerson and Haley's
methodology for labelling sheep GS in CSF using [y32P]8-azidoATP,
to develop this assay or possibly another, using a
fluorescent probe of ATP binding sites, into a robust
procedure suitable for a routine diagnostic laboratory, and
finally to assess whether the presence of GS in CSF is indeed
a marker of AD.
Additional information
Thesis (Master's Degree (Medical Technology)-- Cape Technikon, Cape Town, 1997
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Assay of glutamine synthetase in cerebrospinal fluid as a specific marker.pdf
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