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Tumour metabolism and radioprotection of normal tissue in BALB/c and CBA mice
Author(s)
De Villiers, Neil Heinrich
Date Issued
1992
Type
Thesis
Publisher
Cape Technikon
Abstract
The steady state in a tumour rapidly changes with its growth and the subsequent deteriorating
blood and nutrient supply. This adaptation in the steady state of the tumour is shown in the
increased lactate dehydrogenase and acid phosphatase activity in the tumour during it's
growth. These alterations in the tumour metabolism places an increased burden on the body
to supply nutrient and to discard the waste products of the tumour. This is demonstrated at
the macroscopic level by the decreasing body weight and food intake when the tumour
burden increases, and also at the metabolic levels by the responses of certain glycolytic and
Cori cycle enzymes. Furthermore three distinct stages were observed in the Cori cycle
response to the influence of the tumour namely, a silent or preclinical stage, a
hypermetabolic stage and a hypo metabolic stage. Although the decreasing body weight
cannot be directly linked to the process of gluconeogenesis, the onset of anorexia appeared
to coincide with the end of the hypermetabolic stage and the beginning of the hypometabolic
stage in gluconeogenesis. This clearly shows that the body's steady state is adversely
affected by the presence of the tumour and that the conditions at the metabolic level seem to
cause the anorexia. Furthermore, it is well known that the success of cancer therapies
depends entirely on the effectiveness ofthe modality to kill the tumour cell and on the ability'
of the host to absorb the damage caused by the modality without being destroyed in the
process itself. The second part of this study demonstrates the radioprotective effects of ATP
at all levels. It is clear from this work that ATP had a bigger influence in protecting the
normal tissue than it had on the tumour tissue. This was demonstrated by the response of
acid phosphatase (AP) and glucose-6-phosphate dehydrogenase (G-6-PDH) in the tumour and
testis. Furthermore, it would seem that ATP has a multifactorial interaction with the cell,
two possible mechanisms of protection are indicated by these results. The first of these interactions is through the receptors of the cell to stimulate enhanced glycolysis, for higher
energy production and thus repair. The second possibility is the interaction of ATP with the
receptor of the cell to inhibit the production of free radicals and thus damage, as
demonstrated by the response of G-6-PDH and AP.
blood and nutrient supply. This adaptation in the steady state of the tumour is shown in the
increased lactate dehydrogenase and acid phosphatase activity in the tumour during it's
growth. These alterations in the tumour metabolism places an increased burden on the body
to supply nutrient and to discard the waste products of the tumour. This is demonstrated at
the macroscopic level by the decreasing body weight and food intake when the tumour
burden increases, and also at the metabolic levels by the responses of certain glycolytic and
Cori cycle enzymes. Furthermore three distinct stages were observed in the Cori cycle
response to the influence of the tumour namely, a silent or preclinical stage, a
hypermetabolic stage and a hypo metabolic stage. Although the decreasing body weight
cannot be directly linked to the process of gluconeogenesis, the onset of anorexia appeared
to coincide with the end of the hypermetabolic stage and the beginning of the hypometabolic
stage in gluconeogenesis. This clearly shows that the body's steady state is adversely
affected by the presence of the tumour and that the conditions at the metabolic level seem to
cause the anorexia. Furthermore, it is well known that the success of cancer therapies
depends entirely on the effectiveness ofthe modality to kill the tumour cell and on the ability'
of the host to absorb the damage caused by the modality without being destroyed in the
process itself. The second part of this study demonstrates the radioprotective effects of ATP
at all levels. It is clear from this work that ATP had a bigger influence in protecting the
normal tissue than it had on the tumour tissue. This was demonstrated by the response of
acid phosphatase (AP) and glucose-6-phosphate dehydrogenase (G-6-PDH) in the tumour and
testis. Furthermore, it would seem that ATP has a multifactorial interaction with the cell,
two possible mechanisms of protection are indicated by these results. The first of these interactions is through the receptors of the cell to stimulate enhanced glycolysis, for higher
energy production and thus repair. The second possibility is the interaction of ATP with the
receptor of the cell to inhibit the production of free radicals and thus damage, as
demonstrated by the response of G-6-PDH and AP.
Additional information
Thesis (BTech (Biomedical Technology))--Cape Technikon, 1992.
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97002752_de Villiers_NH_Mtech_Bio_Tech_HWSci_1992_97002752.pdf
Description
Thesis
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