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Typing of human papillomavirus in Western Cape women with cervical intraepithelial neoplasia
Author(s)
Kay, Patti Sheryl
Date Issued
2002
Type
Thesis
Publisher
Cape Technikon
Abstract
Infection \\'ith specific high risk human papilloma"iruses (HPV) has been shown to play a causal
role in the development of ceJVical intraepithelial neoplasia (CIN) and cenical cancer in women.
The development of a prophylactic vaccine to immull.ize women against HPV infection would
play a \'ita! role in protecting women against HPV infection and ultimately ceMcal cancer.
Despite cancer of the cer\'ix being the second most common cancer in South African women, a
literature search reveals that few studies have been performed in South Africa on the types of
HPV prevalent in women with CIN or cancer ofthe ceMx.
HPVs that infect the anogenital tract have also been shown to infect the oral ca\'ity. However, the
HPV prevalence rates vary greatly between studies and the significance of the presence ofHPV
in the oral ca\'ity is still not understood.
The primary objectives of this study were to establish the HPV prevalence rate infecting women
with CIN lesions using a sensitive nested polymerase chain reaction (PCR) and to develop a
novel restriction fragment length polymorphism (RFLP) method to type the high risk mucosal
HPVs detected in these women. The secondary objective of this study was to establish the
prevalence rate and HPV types infecting the oral mucosa of women with CIN lesions and to
compare these HPV types with those detected in the ceMx.
Cemcal punch biopsies were taken from 163 women with CIN lesions and buccal cells were
collected from 33 of these participants. DNAwas extracted from the biopsies and buccal samples and PCR using CCRS primers performed to ensure sample adequacy. Nested PCR usmg
consensus degenerate primers for HPV was performed on all samples sho\\'wg sufficient
amplifiable DNA A novel restriction fragment length pol)morphism (RFLP) method was
developed to identify the 10 high risk mucosal HPVs considered human carcinogens of group 1
by the International Agency for Research on Cancer (lARC) as well as HPV 11 which is
commonly found in the oral cavity.
role in the development of ceJVical intraepithelial neoplasia (CIN) and cenical cancer in women.
The development of a prophylactic vaccine to immull.ize women against HPV infection would
play a \'ita! role in protecting women against HPV infection and ultimately ceMcal cancer.
Despite cancer of the cer\'ix being the second most common cancer in South African women, a
literature search reveals that few studies have been performed in South Africa on the types of
HPV prevalent in women with CIN or cancer ofthe ceMx.
HPVs that infect the anogenital tract have also been shown to infect the oral ca\'ity. However, the
HPV prevalence rates vary greatly between studies and the significance of the presence ofHPV
in the oral ca\'ity is still not understood.
The primary objectives of this study were to establish the HPV prevalence rate infecting women
with CIN lesions using a sensitive nested polymerase chain reaction (PCR) and to develop a
novel restriction fragment length polymorphism (RFLP) method to type the high risk mucosal
HPVs detected in these women. The secondary objective of this study was to establish the
prevalence rate and HPV types infecting the oral mucosa of women with CIN lesions and to
compare these HPV types with those detected in the ceMx.
Cemcal punch biopsies were taken from 163 women with CIN lesions and buccal cells were
collected from 33 of these participants. DNAwas extracted from the biopsies and buccal samples and PCR using CCRS primers performed to ensure sample adequacy. Nested PCR usmg
consensus degenerate primers for HPV was performed on all samples sho\\'wg sufficient
amplifiable DNA A novel restriction fragment length pol)morphism (RFLP) method was
developed to identify the 10 high risk mucosal HPVs considered human carcinogens of group 1
by the International Agency for Research on Cancer (lARC) as well as HPV 11 which is
commonly found in the oral cavity.
Additional information
Thesis (MTech (Biomedical Technology))--Cape Technikon, Cape Town, 2002
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