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The influence of superoxide and anti-oxidants on human sperm function and apoptosis with special reference to the role of red palm oil
Author(s)
Aboua, Yapo Guillaume
Date Issued
2006
Type
Thesis
Publisher
Cape Peninsula University of Technology
Abstract
Cells living under aerobic conditions constantly face the oxygen paradox i.e. oxygen is
indispensable for supporting life; however, its metabolites such as reactive oxygen
species (ROS) can modify cell function. Oxidative stress (OS) arises as a consequence
of excessive ROS production and/or impaired antioxidant defence mechanisms.
Environmental and physiological factors have been implicated in poor sperm function.
Excessive ROS generation results in oxidative damage and consequently decreased
sperm function. The objectives of this study are threefold: (i) To measure the
production of O
2
-. by sperm by means of flow cytometry. (ii) To determine effects of
O2
-. on sperm motility and viability in the presence or absence of commercially
available scavengers and RPO. (iii) To determine effects of O2
-. on selective apoptotic
markers in ejaculated sperm in the presence of absence of commercially available
scavengers and RPO.
In the first part of the study, suitable solvents were investigated in order to introduce
RPO (because of its hydrophobic nature) as a possible scavenger of ROS in human
spermatozoa. Secondly, the O2
-. donor; 2, 3-dimetoxy-1-naphthoquinone (DMNQ)
(2.5~M-100~M, 60 min.) was added to normozoospermic post swim-up samples in the
absence or presence of Mn(lIl)TMPyP (50~M) or SOD (501U) or RPO at 0.1% and
0.5%. CASA was used to analyse motility parameters, while FACS was used to
determine viability (PI, 1mM, 15 min.) and O2
-. levels (DHE, 30~M, 15min.).
indispensable for supporting life; however, its metabolites such as reactive oxygen
species (ROS) can modify cell function. Oxidative stress (OS) arises as a consequence
of excessive ROS production and/or impaired antioxidant defence mechanisms.
Environmental and physiological factors have been implicated in poor sperm function.
Excessive ROS generation results in oxidative damage and consequently decreased
sperm function. The objectives of this study are threefold: (i) To measure the
production of O
2
-. by sperm by means of flow cytometry. (ii) To determine effects of
O2
-. on sperm motility and viability in the presence or absence of commercially
available scavengers and RPO. (iii) To determine effects of O2
-. on selective apoptotic
markers in ejaculated sperm in the presence of absence of commercially available
scavengers and RPO.
In the first part of the study, suitable solvents were investigated in order to introduce
RPO (because of its hydrophobic nature) as a possible scavenger of ROS in human
spermatozoa. Secondly, the O2
-. donor; 2, 3-dimetoxy-1-naphthoquinone (DMNQ)
(2.5~M-100~M, 60 min.) was added to normozoospermic post swim-up samples in the
absence or presence of Mn(lIl)TMPyP (50~M) or SOD (501U) or RPO at 0.1% and
0.5%. CASA was used to analyse motility parameters, while FACS was used to
determine viability (PI, 1mM, 15 min.) and O2
-. levels (DHE, 30~M, 15min.).
Additional information
Thesis (MTech (Biomedical Technology))--Cape Peninsula University of Technology, 2006
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