Loading...
Modelling of a bioflocculant supported dissolved air flotation system for fats oil and grease laden wastewater pretreatment
Author(s)
Mukandi, Melody
Date Issued
2017
Type
Thesis
Publisher
Cape Peninsula University of Technology
Abstract
In the recent past, the poultry industry in South Africa has grown due to an increased demand of
poultry products as a result of population growth and improved living standards. Furthermore,
this has led to poultry slaughterhouses generating high strength wastewater which is laden with
a high concentration of organic and inorganic pollutants from the slaughtering process and
sanitation of equipment and facilities. As a result, South Africa has promulgated restrictions and
a set of quality standards for effluent discharged into the environment to minimize ecological
degradation and human health impact. Hence, there is a need for improved Poultry
Slaughterhouse Wastewater (PSW) pre-treatment prior to either discharge into municipal
wastewater treatment plants (WWTP) or on-site secondary treatment processes such as
anaerobic digesters. Additionally, amongst the pre-treatment methods for Fats, Oil and Grease
(FOG) laden wastewater, flotation remains the most popular with Dissolved Air Flotation (DAF)
system being the most applied. However, modelling and optimization of a biological DAF system
has never been attempted before in particular for a bioflocculant supported DAF (BioDAF) for
PSW pre-treatment. Process modelling and optimization involves process adjustment to
optimize influential parameters. In this study, Response Surface Methodology (RSM) was used
to develop an empirical model of a BioDAF for pre-treatment of PSW, for which a bioflocculant
producer including production conditions, flocculant type and its floc formation mechanism, were
identified.
Twenty-one (n = 21) microbial strains were isolated from the PSW and their flocculation activity
using kaolin clay suspension (4g/L) was quantified, with a mutated Escherichia coli (mE.coli)
[accession number LT906474.1], having the highest flocculation activity even in limited nutrient
conditions; hence, it was used for further analysis in other experiments. Furthermore, the
optimum conditions for bioflocculant production achieved using RSM were pH of 6.5 and 36°C
conditions which induced instantaneous bioflocculant production with the highest flocculation
activity. The bioflocculant produced by the mE.coli showed the presence of carboxyl/amine,
alkyne and hydroxyl functional groups, which was indicative that the bioflocculant contained
both polysaccharides and some amino acids.
poultry products as a result of population growth and improved living standards. Furthermore,
this has led to poultry slaughterhouses generating high strength wastewater which is laden with
a high concentration of organic and inorganic pollutants from the slaughtering process and
sanitation of equipment and facilities. As a result, South Africa has promulgated restrictions and
a set of quality standards for effluent discharged into the environment to minimize ecological
degradation and human health impact. Hence, there is a need for improved Poultry
Slaughterhouse Wastewater (PSW) pre-treatment prior to either discharge into municipal
wastewater treatment plants (WWTP) or on-site secondary treatment processes such as
anaerobic digesters. Additionally, amongst the pre-treatment methods for Fats, Oil and Grease
(FOG) laden wastewater, flotation remains the most popular with Dissolved Air Flotation (DAF)
system being the most applied. However, modelling and optimization of a biological DAF system
has never been attempted before in particular for a bioflocculant supported DAF (BioDAF) for
PSW pre-treatment. Process modelling and optimization involves process adjustment to
optimize influential parameters. In this study, Response Surface Methodology (RSM) was used
to develop an empirical model of a BioDAF for pre-treatment of PSW, for which a bioflocculant
producer including production conditions, flocculant type and its floc formation mechanism, were
identified.
Twenty-one (n = 21) microbial strains were isolated from the PSW and their flocculation activity
using kaolin clay suspension (4g/L) was quantified, with a mutated Escherichia coli (mE.coli)
[accession number LT906474.1], having the highest flocculation activity even in limited nutrient
conditions; hence, it was used for further analysis in other experiments. Furthermore, the
optimum conditions for bioflocculant production achieved using RSM were pH of 6.5 and 36°C
conditions which induced instantaneous bioflocculant production with the highest flocculation
activity. The bioflocculant produced by the mE.coli showed the presence of carboxyl/amine,
alkyne and hydroxyl functional groups, which was indicative that the bioflocculant contained
both polysaccharides and some amino acids.
Additional information
Thesis (MTech (Chemical Engineering))--Cape Peninsula University of Technology, 2017.
File(s)![Thumbnail Image]()
Loading...
Name
211050458-Mukandi-Melody-MEng-Chemical-Engineering-Eng-2018.pdf
Description
Thesis
Size
1.78 MB
Format
Adobe PDF
Checksum
(MD5):343045869d0b7063b3bc3fc762058009