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Prophylaxis against adenovirus pneumonia : a laboratory investigation
Author(s)
Lambrick, Maureen
Date Issued
1991
Type
Thesis
Publisher
Cape Technikon
Abstract
Adenovirus type 7 (Ad?) is the sub-species of adenovirus most frequently associated with
serious illness. In Cape Town, the strain predominantly responsible for infantile
pneumonia due to adenovirus is adenovirus type 7c (Ad7c), and it has been the cause of
several outbreaks of nosocomial infection in the respiratory unit at the Red Cross War
Memorial Hospital for Children..
This study explored two strategies which could possibly assist in the prevention of the
spread of adenovirus infection in the hospital environment.
The guanosine analog, 9-(1,3-dihydroxy-2-propoxymethyl) guanine (DHPG) is a highly
effective antiviral agent against herpesviruses. It has also been reported that this drug
could have therapeutic potential with regard to adenovirus infections. Based on this
postulate, it was decided to perform in vitro studies to evaluate the antiviral activity of
DHPG against Ad7c.
DHPG was tested for it's inhibitory potential against the replication of Ad7c in cell
culture. The virus was propagated in the presence of drug concentrations ranging from 2
pM to 150 pM and the manifestation of cytopathic effect was monitored. Ad7c
replication in cell culture was not inhibited by the drug at those concentrations that were
tested, although growth ~f herpes simplex virus, used as a control, was significantly
impaired.
The value of passive immunization in the prevention or amelioration of disease has been
well recognized. Commercial preparations of immunoglobulin are not routinely
monitored for the presence of antibodies to Ad7c. In this study, 17 batches of
intramuscular and intravenous globulin were tested for the presence of neutralizing
antibodies specific for Ad7c. The batches, which were randomly selected, were all
found to contain high levels of neutralizing antibodies to Ad7c.
The conclusions reached were that DHPG will not be an efficacious antiviral agent for
treatment of Ad7c infections, but that the implementation of passive immunization to
children who are infected or exposed to Ad7c in a hospital ward, may be of help to
control the spread of nosocomial infections.
serious illness. In Cape Town, the strain predominantly responsible for infantile
pneumonia due to adenovirus is adenovirus type 7c (Ad7c), and it has been the cause of
several outbreaks of nosocomial infection in the respiratory unit at the Red Cross War
Memorial Hospital for Children..
This study explored two strategies which could possibly assist in the prevention of the
spread of adenovirus infection in the hospital environment.
The guanosine analog, 9-(1,3-dihydroxy-2-propoxymethyl) guanine (DHPG) is a highly
effective antiviral agent against herpesviruses. It has also been reported that this drug
could have therapeutic potential with regard to adenovirus infections. Based on this
postulate, it was decided to perform in vitro studies to evaluate the antiviral activity of
DHPG against Ad7c.
DHPG was tested for it's inhibitory potential against the replication of Ad7c in cell
culture. The virus was propagated in the presence of drug concentrations ranging from 2
pM to 150 pM and the manifestation of cytopathic effect was monitored. Ad7c
replication in cell culture was not inhibited by the drug at those concentrations that were
tested, although growth ~f herpes simplex virus, used as a control, was significantly
impaired.
The value of passive immunization in the prevention or amelioration of disease has been
well recognized. Commercial preparations of immunoglobulin are not routinely
monitored for the presence of antibodies to Ad7c. In this study, 17 batches of
intramuscular and intravenous globulin were tested for the presence of neutralizing
antibodies specific for Ad7c. The batches, which were randomly selected, were all
found to contain high levels of neutralizing antibodies to Ad7c.
The conclusions reached were that DHPG will not be an efficacious antiviral agent for
treatment of Ad7c infections, but that the implementation of passive immunization to
children who are infected or exposed to Ad7c in a hospital ward, may be of help to
control the spread of nosocomial infections.
Additional information
Thesis (Masters Diploma Technology (Medical Technology)) -- Cape Technikon, Cape Town, 1991
Subjects
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