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The role of monoclonal antibodies in the diagnosis of acute leukaemia
Author(s)
McLellan, Gail
Date Issued
1990
Type
Thesis
Publisher
Cape Technikon
Abstract
Eighty six patients with acute l.eukaemia were studied using
morphol.ogical., cytochemical. and immunol.ogical. techniques. The
acute l.eukaemias were subdivided using the
French-American-British (FAB) cl.assification. The
immunophenotyping studies were compared with the morphological
classification to assess their contribution to the diagnosis.
Acute non-lymphoblastic leukaemia (ANLL) was diagnosed on the
basis of morphol.ogy and cytochemical. criteria. In addition this
group of patients was studied with antibodies directed against
myel.omonocytic antigens. However, no further cl.inical.l.y useful.
information was obtained. Patients whose bl.asts did not stain
with Sudan black or myel.operoxidase were considered to have
acute lymphoblastic leukaemia (ALL). After assessment with
monocl.onal. antibodies directed against epitopes expressed on
cel.l.s from the l.ymphoid lineage, these patients were subgrouped
into non-T-ALL, common-ALL, B-ALL, T-ALL and l.ymphoblastic
lymphoma categories. This study confirmed the val.ue of
monocl.onal. antibodies for accurately assigning l.ineage to the
acute l.eukaemias and particularly in those situations where
conventional morphol.ogical. criteria and cytochemical. markers
are inconclusive.
morphol.ogical., cytochemical. and immunol.ogical. techniques. The
acute l.eukaemias were subdivided using the
French-American-British (FAB) cl.assification. The
immunophenotyping studies were compared with the morphological
classification to assess their contribution to the diagnosis.
Acute non-lymphoblastic leukaemia (ANLL) was diagnosed on the
basis of morphol.ogy and cytochemical. criteria. In addition this
group of patients was studied with antibodies directed against
myel.omonocytic antigens. However, no further cl.inical.l.y useful.
information was obtained. Patients whose bl.asts did not stain
with Sudan black or myel.operoxidase were considered to have
acute lymphoblastic leukaemia (ALL). After assessment with
monocl.onal. antibodies directed against epitopes expressed on
cel.l.s from the l.ymphoid lineage, these patients were subgrouped
into non-T-ALL, common-ALL, B-ALL, T-ALL and l.ymphoblastic
lymphoma categories. This study confirmed the val.ue of
monocl.onal. antibodies for accurately assigning l.ineage to the
acute l.eukaemias and particularly in those situations where
conventional morphol.ogical. criteria and cytochemical. markers
are inconclusive.
Additional information
Thesis (Master Diploma (Medical Technology)) -- Cape Technikon, Cape Town, 1990
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