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The effect of red palm oil supplementation of an oxidative risk induced diet and a high saturated fat diet on ischaemia/perfusion injury in the isolated perfused rat heart
Author(s)
Bester, Dirk Jacobus
Date Issued
2006
Type
Thesis
Publisher
Cape Peninsula University of Technology
Abstract
Research has shown that the activation of the NO-cGMP pathway leads to
myocardial protection from oxidative stress conditions, such as ischaemia and
reperfusion. Few of these studies have however combined diet induced oxidative
stress with ischaemia/reperfusion injury. Although little is known about the effects
of supplements such as red palm oil (RPO) on the NO-cGMP pathway, research
has shown that dietary RPO-supplementation improved reperfusion aortic output
recovery through mechanisms that may include activation of the NO-cGMP- and
inhibition of the cAMP pathway. RPO is an antioxidant-rich oil containing ~carotene
and Vitamin E (tocopherols and tocotrienols). The aims of this study
were to determine: 1) whether RPO-supplementation of an oxidative risk induced
diet (ORD) and a high saturated fat diet (HFD) offers protection against
ischaemia/reperfusion injury in the isolated perfused rat heart and 2) the possible
mechanisms for this protection.
Male Wistar rats were randomly divided into four groups for a period of 14 weeks
according to the dietary supplementation they received. The control groups
received either an oxidative risk induced diet (ORD) or a high saturated fat diet
(HFD), while the experimental groups received an ORD supplemented with RPO
(ORD+RPO) or a HFD supplemented with RPO (HFD+RPO).
myocardial protection from oxidative stress conditions, such as ischaemia and
reperfusion. Few of these studies have however combined diet induced oxidative
stress with ischaemia/reperfusion injury. Although little is known about the effects
of supplements such as red palm oil (RPO) on the NO-cGMP pathway, research
has shown that dietary RPO-supplementation improved reperfusion aortic output
recovery through mechanisms that may include activation of the NO-cGMP- and
inhibition of the cAMP pathway. RPO is an antioxidant-rich oil containing ~carotene
and Vitamin E (tocopherols and tocotrienols). The aims of this study
were to determine: 1) whether RPO-supplementation of an oxidative risk induced
diet (ORD) and a high saturated fat diet (HFD) offers protection against
ischaemia/reperfusion injury in the isolated perfused rat heart and 2) the possible
mechanisms for this protection.
Male Wistar rats were randomly divided into four groups for a period of 14 weeks
according to the dietary supplementation they received. The control groups
received either an oxidative risk induced diet (ORD) or a high saturated fat diet
(HFD), while the experimental groups received an ORD supplemented with RPO
(ORD+RPO) or a HFD supplemented with RPO (HFD+RPO).
Additional information
Thesis (MTech (Biomedical Technology))--Cape Peninsula University of Technology, 2006
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