Effect of smoking and waist circumference on biochemical markers of oxidative stress in subjects with IGT and newly diagnosed diabetics from Bellville South, Western Cape, South Africa

Abstract

Studies have shown that oxidative stress (OS) is a major pathological risk factor in various diseases, including type 2 diabetes mellitus (T2DM). Hyperglycemia independently is a generator of free radicals, hence increases the level of OS in T2DM subjects. The oxidation of LDL is suggested to play a significant role in the pathogenesis of macrovascular complications observed in diabetic patients. In subjects with hyperglycemia or normoglycemia we investigated the relationship between MDA-protein adducts, HNE-protein adducts and auto-antibodies against oxLDL, and cardiovascular profile as measured by hs- CRP. From an epidemiological study that screened a high risk urban population for diabetes using oral glucose tolerance test, 98 hyperglycaemie and 79 normoglycaemic individuals were selected for this study. Enzyme linked immuno-sorbent-assay methods were used to determine the levels of serum MDA-protein adducts, HNE-protein adducts or auto-antibodies against oxLDL. High sensitive CRP was measured by nephelometry.